Olea25® Hydroxytyrosol Plant Based Polyphenolic Compound
100% Pharmaceutical Food Grade Polyphenols ~ 25% Bioactive Hydroxytyrosol
Olea 25 ® ~ Combats Five Predominant Reactive Species
Antioxidant Power Against Peroxyl Radicals 15,710 μmole TE/gram Antioxidant Power Against Hydroxyl Radicals 24,100 μmole TE/gram Antioxidant Power Against Peroxynitrite 831 μmole TE/gram Antioxidant Power Against Super Oxide Anion 25,445 μmole TE/gram Antioxidant Power Against Singlet Oxygen 2,490 μmole TE/gram 68,576 μmole TE/gram ~ Total (ORAC) 6,857,600 μmole TE/100 gram *Brunswick Laboratories μmoleTE/100g Highest Antioxidant Discovered to Date Worldwide
Research Of Olea Europaea (Olive Tree) Ancient Medicinal Properties Of Leaves & Fruit
Ezekiel 47:12 “By the river on its bank, on one side and on the other, will grow all kinds of trees for food. Their leaves will not wither and fruit will not fail. They will bear every month, because their water flows from the sanctuary and their fruit will be for food and their leaves for healing.”
The medicinal properties of the leaves and fruit of Olea Europaea (olive tree) have been known since antiquity. Numerous contemporary studies have linked the Mediterranean diet with increased optimum health. In particular, consumption of olive oil has been associated with a decreased risk of cardiovascular disease and certain cancers. Increasingly, there has been an interest in the biological properties of polyphenols, which are minor constituents of olive oil. For example, hydroxytyrosol has been shown to be a potent antioxidant and has anti-atherogenic and anti-cancer properties. The overall aim of this study was to provide insights into the molecular mechanisms of hydroxytyrosol using genome-wide mRNA-Seq. Initial experiments were aimed at assessing cytotoxicity, apoptosis and cell cycle effects of hydroxytyrosol in various cell lines.
The findings indicated a dose-dependent reduction in cell viability in human erythroleukemic K562 and human keratinocytes. When comparing the viability in parental CEM-CCRF and R100 cells (which overexpress the P-glycoprotein pump), it was determined that the R100 cells were more resistant to effects of hydroxytyrosol suggesting efflux by the multi-drug resistance pump. By comparing the uptake of Hoechst 33342 in the two cell lines that had been pretreated with hydroxytyrosol, it was determined that the polyphenol may have P-glycoprotein-modulating activity. Further, initial studies indicated modest radioprotective effects of relatively low doses of hydroxytyrosol in human keratinocytes. Analysis of mRNA sequencing data identified that treatment of keratinocytes with 20 μM hydroxytyrosol results in the upregulation of numerous antioxidant proteins and enzymes, including heme oxygenase-1 (15.46-fold upregulation), glutaredoxin (1.65) and glutathione peroxidase (1.53).
This may account for the radioprotective activity of the compound, and reduction in oxidative stress suggests a mechanism for chemoprevention of cancer by hydroxytyrosol. Alteration in the expression of transcription factors may also contribute to the anti-cancer effects described in numerous studies. These include changes in the expression of STAT3, STAT6, SMAD7 and ETS-1. The telomerase subunit TERT was also found to be downregulated in K562 cells. Overall, our findings reveal that hydroxytyrosol is the highest antioxidant discovered to date worldwide. Hydroxytyrosol nourishes all cells, tissues and organs from within by combating free radical scavengers.